|
Study Number: |
2004 |
|---|---|
|
Project Title: |
Microbial biomass. |
|
Investigator(s): |
David C. Coleman
Biographical Sketch
(Retired) Christina Wright Note: Researcher is retired. For further information regarding this project, please contact: Coweeta LTER Coweeta Hydrologic Laboratory 3160 Coweeta Lab Road Otto, North Carolina 28763 USA Telephone: 828.524.2128 | FAX: 828.369.6768 |
| Affiliated Institution(s): | University of Georgia |
| Address: |
University of Georgia Ecology Annex Athens Georgia 30602-2360 USA |
| Study Category: | Riparian |
| Project Type: | Type 1 |
| Study Period: |
03/1994 - 12/1994. |
| Status/Notes: |
|
Funding Source(s): |
National Science Foundation, Grants DEB-9632854 (Text Version) and DEB-0218001 (Text Version) to Coweeta LTER. |
| Abstract: |
All of the data originated in the
riparian watershed (WS 55) at Coweeta. Thus
far, all soil data occurred in 1994 - ie. before the rhododendron
removal. The
summary C and N files are saved by date (year, month,and day). Within
each file
are data for Microbial carbon and nitrogen. The sample names (ex. C1-1,
0-5)
indicate control plot, 1 meter distance from the stream, sample number
1, at the
0-5 cm soil depth, and so on. T would indicate treatment plot and 5-10
indicates
the 5-10 cm soil depth. Within each plot (C and T), we established three transects, 10 meters in length, at 1, 5, and 15 meter distances upslope from a stream. Along each transect, 4 samples were collected and each sample consisted of two bulked soil cores, separated by depth (0-5 cm and 5-10 cm). All sampling dates were in 1994, beginning in March. Sample dates were selected to correspond with seasonal changes and occurred March 25, June 16, August 16, November 1, and December 16. Microbial C data is listed as microgram C per gram dry weight soil, followed by an average of C (measured by distance from the stream) and standard deviation corresponding to that average. ND listed in the Project indicates that the sample needs to be reanalyzed or was missing. The december Project needs more work, but I have included it anyway. Microbial nitrogen is listed as microgram of total persulfate N per gram dry weight soil. This is again followed by an average N value by distance from the stream and depth of the soil and the corresponding standard deviation. |
| Location(s), Described: | Watershed 55. |
| Location(s), Download GPS: |
ArcView Shape Files (shp.): UTM,
NAD83, Zone 17 |
Lat/Lon Note: Files originated from Project 2007 conducted at Watershed 55. |
| Location(s), Online Map(s): |
 USGS Topographic-based Maps of Research Sites(Printable for fieldwork) Note: Map originated from Project 2007 conducted at Watershed 55. |
| Methods/Experimental Design: |
Methods: Microbial Biomass C & N Chloroform fumigation - extraction method was used to determine microbial biomass carbon and nitrogen (Vance et al., 1987). All samples were extracted with 0.5 M K2SO4 for 30 minutes and all extracts were filtered through Whatman 42 filter paper and stored frozen until analyzed for microbial C and N. Microbial carbon was determined using a Shimadzu TOC-500 Total C analyzer to directly analyze the extracts as described above. Data are reported as microgram total organic carbon (TOC) per gram dry weight soil. The correction factor used for carbon was kc = 0.33 (Smith and Paul, 1990). Microbial nitrogen was determined by the total persulfate N (TPN) protocol of Cabrera and Beare, 1993. In this protocol, an aliquot of extract was first digested with a persulfate oxidizing reagent and then analyzed using an Alpkem RFA 300 colorimetric spectrophotometer. Data are reported as microgram TPN per gram dry weight soil. The correction factor for nitrogen was kn = 0.38 (Voroney and Paul, 1984). Collected as described above and then refrigerated until all samples were picked free of roots and recognizable plant material.
Variable] [Coded Value]
[Definition] |
| Sampling Frequency: | 1994: March 25, June 16, August 16, November 1, December 16. |
| Data Columns: |
ID - Record identification
number. Date - YY/MM/DD Site - Site Carbon - See abstract. Nitrogen - See abstract. Missing Data Codes: . (Period) |
| Publications: |
Wright, Christina Jane. 1998. The effects of man-made and natural disturbance events upon soil microbial biomass, soil fauna, soil nutrient cycling, and litter decomposition in the southern Appalachians. Ph.D. Dissertation. University of Georgia. |
| Data Restrictions: | Users must adhere to the Coweeta LTER Data Policy. |
| Metadata: | EML Format (XML Schema) | Information about EML |
| Data Downloads: |
Microsoft® Excel (.xls) Text Comma Delimited (.csv) DBase (.dbf) |