Study Number: 

1069

Project Title:

Examining the potential effects of phytophagous insect frass on forest nitrogen cycling.

Investigator(s):

Mark D. Hunter  Biographical Sketch  (Retired)
Bradley Balukjian


Note: Researcher is retired. For further information regarding this project, please contact:

Coweeta LTER
Coweeta Hydrologic Laboratory
3160 Coweeta Lab Road
Otto, North Carolina 28763 USA
Telephone: 828.524.2128 | FAX: 828.369.6768
Affiliated Institution(s): University of Georgia
Address: Institute of Ecology
University of Georgia
Athens, Georgia 30602  USA
Study Type: Terrestrial
Project Type: Type 1
Study Period:

05/1999 - 12/2001

Status/Notes:

Graduate Research

Funding Sources:

National Science Foundation, DEB-9632854 (Text Version) and DEB-0218001 (Text Version) to Coweeta LTER.

Abstract: Human alteration of the global nitrogen cycle has increased the importance of understanding the major sources, sinks, and fluxes of the cycle within natural ecosystems. One poorly understood component of the nitrogen cycle in forest ecosystems is the contribution of phytophagous insect frass on soil N-cycling dynamics. This study proposes to investigate the influence of frass on (1) the dynamics of soil N-cycling; (2) the loss of N from the ecosystem via leaching; (3) the slow cycle decomposition of post-herbivore leaf litter; and (4) the overall potential of soil near the Coweeta Hydrologic Laboratory to retain nitrogen. Red Oak (Quercus rubra [Fagaceae]) saplings will be subjected to defoliation by the seasonal phytophagous insect larvae of the White-Marked Tussock Moth (Orgyia leucostigma [Lepidoptera: Lymantriidae]) and the frass added to the soil in a factorial, controlled experimental  design. Soil nutrient concentrations, leachate nutrient concentrations, "soil" respiration, and leaf quality will be measured. The project is expected to increase our understanding of the complex relationship between above-ground and below-ground nitrogen cycling dynamics as well as inform the current debate concerning the relative impacts and importance of human versus natural alterations to the nitrogen cycle.


Resources for students about terms used in this study:
The nitrogen cycle - Source: Visionlearning
Red oak - Source: USDA Plants Database
Whitemarked Tussock Moth - Source: forestpests.org
Location(s), Described: In watershed 27, approximately .5km past the gate at the Pickin's Nose trail head, there is what appears to be an abandoned roadway with a steep entrance. The "road" leads to the remnants of a campfire site just after the entrance. North of the small camp clearing there is a downed tree and an area with canopy cover but no trees.
Location(s), Download GPS: ArcView Shape Files (shp.):  UTM, NAD83, Zone 17  |  Lat/Lon
Location(s), Online Maps: Online Map
Methods/Experimental Design: We are conducting a microcosm experiment to attempt to replicate Barbara Reynolds' results of a NO3 pulse in the soil and catchment drainage stream following a Periclista sp. outbreak (Reynolds, Barbara, C.; Hunter, Mark D.; Crossley, D.A., Jr. 2000. Effects of Canopy Herbivory On Nutrient Cycling In A Northern Hardwood Forest In Western North Carolina. Selbyana. 21(1.2): 74-78).

We are using Quercus rubra saplings and Orgyia leucostigma (White Marked Tussock Moths) in the experimental system, and will be measuring nitrate concentrations in the soil and leachate. In order to closely replicate field conditions, we would like to remove a relatively small amount of surface litter and soil from a high-elevation site near Kitti's high elevation site.  Saplings were shipped in 3-gallon pots and will be potted up to 7-gallon pots for the experiment. There are 8 treatments with 20 replicates/treatment, for a total of 160 trees. Each pot has a volume of approximately .34 m^3, and approximately half of each pot will require soil, as each sapling is in a potting soil mix currently. From these calculations, it is estimated that approximately 2.046 m^3 of soil and surface litter will be required.

Sampling Frequency: A single removal period that will be broken into two or three consecutive trips.
Data Columns:

Date - Date (month-year)
Elevation - Elevation; meters (m)
Replicate - Replicate number
Frass Weight - Frass weight; grams (g)
Frass Nitrogen - Frass nitrogen; grams (g)
Frass Carbon - Frass carbon; grams (g)

Publications:

Frost, Christopher J.; Hunter, Mark D. 2004. Insect canopy herbivory and frass deposition affect soil nutrient dynamics and export in oak mesocosms. Ecology. 85(12): 3335-3347.

Data Restrictions: Users must adhere to the Coweeta LTER Data Policy.
Metadata: EML Format (XML Schema) | Information about EML
Data Downloads: Microsoft® Excel (.xls)
Text Comma Delimited (.csv)
DBase (.dbf)